Effect of Medium Salt Strength on the Micropropagation, Phenolic Content and Antioxidant Activity of Arnica montana L., Threatened Plant Species

Abstract

Author(s): Ely Zayova, Maria Petrova, Milena Nikolova and Lyudmila Dimitrova

The effect of different strength of Murashige and Skoog (MS) medium (full, half-, thirdand quarter content of salts and vitamins) on micropropagation, shoot growth, in vitro rooting and antioxidant properties of Arnica montana was demonstrated. The full strength MS medium supplemented with 1 mg L-1 6-benzylaminopurine (BAP) and 0.1 mg L-1 indole-3-acetic acid (IAA) was the best for plant multiplication (11.4 number of shoots per explant) and biomass production (FW 1.66 g and DW 0.25 g). Optimal rooting medium was half strength MS rooting medium contained 0.5 mg L-1 indole-3-butyric acid (IBA), although the maximum number of roots was induced on quarter strength MS rooting medium. The rooted plants were successfully ex vitro acclimatized. The highest phenolic content and antioxidant activity were recorded in shoots grown on ¼ strength of MS medium, whether derived from micropropagation or from rooting stage. This study showed that tissue culture of A. montana is alternative source of natural antioxidants

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